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KMID : 0358320070480050522
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Korean Journal of Urology
2007 Volume.48 No. 5 p.522 ~ p.526
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Changes in the Expression of Smooth Muscle Myosin Heavy Chain mRNA following Partial Bladder Obstruction or Spinal Cord Injury in Rat: A Preliminary Study
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Byun Young-Min
Lee Gil-Ho
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Abstract
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Purpose: The smooth muscle myosin heavy chain(SMMHC) isoform composition has been actively researched in a partial bladder obstruction (PBO) or spinal cord injury(SCI) model. Even though rat is an ideal animal for studying bladder physiology, there were very few reports about the changes of the SMMHC isoforms in the PBO or SCI injured bladder of rat. We developed two polymerrase chain reaction(PCR) primer sets to amplify the isoforms and we applied the primers to the PBO and SCI rat models.
Materials and Methods: Female rats had their bladder necks surgically obstructed or they were subjected to spinal cord injury. Six weeks after the event, the bladders were excised. The expression of the C-terminal (SM1 and SM2) and N-terminal(SM-A and SM-B) isoforms of SMMHC was analyzed by performing reverse transcriptase-PCR(RT-PCR).
Results: The control bladder showed only the SM-B isoform in the C-terminal. However, the bladder after SCI showed an increased SM-A to SM-B ratio. In case of PBO, the ratios were variable. A decreased SM1 expression was noted in the PBO and SCI groups when compared to the control group(p＜0.05).
Conclusions: Our female rat models for PBO or SCI demonstrates changes in the expression of smooth muscle myosin heavy chain isoforms. We will apply this primer set for studying of rat muscular physiology in PBO or SCI model. (Korean J Urol 2007;48:522-526)
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KEYWORD
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Rats, Myosin heavy chains, Urinary bladder neck obstruction, Spinal cord injuries
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